mmlv reverse transcriptase protocol

173 0 obj <> endobj Fill out our Technical Support Form, The enzyme is used to synthesize first-strand cDNA up to 7 kb. For those product lots not listed below, please contact NEB at, Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. By creating an account, you confirm that you accept the, Plate Readers, Fluorometers & Luminometers, Privacy Policy and Requests for Information. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual.

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both DNase and RNase. M1701, M1705. 0000004531 00000 n This plasmid is available through Addgene. To save your cart and view previous orders, sign in to your NEB account. To protect your privacy, your account has been locked after 6 failed login attempts. M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M …

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Can DNA be used as a template for M-MuLV Reverse Transcriptase? Contents and storage endstream endobj startxref You've created a Promega.com account. There was an error processing your request. 0000107421 00000 n Complete Protocol endstream endobj 174 0 obj <>/Outlines 238 0 R/Metadata 20 0 R/Pages 170 0 R/PageLayout/SinglePage/OpenAction 175 0 R/Type/Catalog>> endobj 175 0 obj <> endobj 176 0 obj <>/MediaBox[0 0 306 396]/Resources<>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]>>/Type/Page>> endobj 177 0 obj <>stream Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity. 0000067035 00000 n (1-4).

0000039826 00000 n There was an issue logging into your account. Please update your browser to Internet Explorer 11 or above. Overview Protocols Specifications Resources Related Products Workflows RNA-Dependent DNA Polymerase for cDNA Synthesis Moloney Murine Leukemia Virus Reverse Transcriptase(MMLV RT,M-MLV 反转录酶)是一种 RNA 依赖的 DNA 聚合酶,可用于长 mRNA(>5kb)为模板的 cDNA 合成。 %PDF-1.6 %���� 0000002873 00000 n

Tested for the absence of DNases and RNases. Get in touch with a nearby distributor or sales representative, Instructions for Use of Product(s) Thank you for verifying your email address. Please enter a quantity for at least one size, One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA), Molecular Cloning: A Laboratory Manual(2nd Ed. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). 0000001829 00000 n 50% Glycerol Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?

ProtoScript® First Strand cDNA Synthesis Kit, ProtoScript® II First Strand cDNA Synthesis Kit, Tested for the absence of DNases and RNases. All Rights Reserved. M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.

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