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both DNase and RNase. M1701, M1705. 0000004531 00000 n This plasmid is available through Addgene. To save your cart and view previous orders, sign in to your NEB account. To protect your privacy, your account has been locked after 6 failed login attempts. M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M …
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Can DNA be used as a template for M-MuLV Reverse Transcriptase? Contents and storage endstream endobj startxref You've created a Promega.com account. There was an error processing your request. 0000107421 00000 n Complete Protocol endstream endobj 174 0 obj <>/Outlines 238 0 R/Metadata 20 0 R/Pages 170 0 R/PageLayout/SinglePage/OpenAction 175 0 R/Type/Catalog>> endobj 175 0 obj <> endobj 176 0 obj <>/MediaBox[0 0 306 396]/Resources<>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]>>/Type/Page>> endobj 177 0 obj <>stream Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity. 0000067035 00000 n (1-4).
0000039826 00000 n There was an issue logging into your account. Please update your browser to Internet Explorer 11 or above. Overview Protocols Specifications Resources Related Products Workflows RNA-Dependent DNA Polymerase for cDNA Synthesis Moloney Murine Leukemia Virus Reverse Transcriptase（MMLV RT，M-MLV 反转录酶）是一种 RNA 依赖的 DNA 聚合酶，可用于长 mRNA（>5kb）为模板的 cDNA 合成。 %PDF-1.6 %���� 0000002873 00000 n
Tested for the absence of DNases and RNases. Get in touch with a nearby distributor or sales representative, Instructions for Use of Product(s) Thank you for verifying your email address. Please enter a quantity for at least one size, One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA), Molecular Cloning: A Laboratory Manual(2nd Ed. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). 0000001829 00000 n 50% Glycerol Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?
ProtoScript® First Strand cDNA Synthesis Kit, ProtoScript® II First Strand cDNA Synthesis Kit, Tested for the absence of DNases and RNases. All Rights Reserved. M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
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